Cellulose acetate as solid phase in ELISA for plague
Mem. Inst. Oswaldo Cruz
; 95(1): 95-6, Jan.-Feb. 2000. graf
Article
em En
| LILACS
| ID: lil-251319
Biblioteca responsável:
BR1.1
ABSTRACT
Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 µg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 14,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Peste
/
Yersinia pestis
/
Celulose
/
Antígenos de Bactérias
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Brasil