Rat hindlimb joint immobilization with acrylic resin orthoses
Braz. j. med. biol. res
; 39(7): 979-985, July 2006. ilus, tab
Article
em En
| LILACS
| ID: lil-431570
Biblioteca responsável:
BR1.1
RESUMO
The objective of the present study was to propose an orthosis of light material that would be functional for the animal and that would maintain only the ankle joint immobilized. Male Wistar rats (3 to 4 months old, 250-300 g) were divided into 2 groups (N = 6) control and immobilized for 7 days. Rats were anesthetized with sodium pentobarbital (40 mg/kg weight) and the left hindlimb was immobilized with the orthoses composed of acrylic resin model, abdominal belt and lateral supports. The following analyses were performed glycogen content of the soleus, extensor digitorum longus, white gastrocnemius, red gastrocnemius, and tibialis anterior muscles by the phenol sulfuric method, and the weight, fiber area and intramuscular connective tissue of the soleus by the planimetric system. Data were analyzed statistically by the Kolmogorov-Smirnov, Student t and Wilcoxon tests. Immobilization decreased glycogen in all muscles (P < 0.05; soleus 31.6 percent, white gastrocnemius 56.6 percent, red gastrocnemius 39 percent, extensor digitorum longus 41.7 percent, tibialis anterior 45.2 percent) in addition to reducing soleus weight by 34 percent (P < 0.05). Furthermore, immobilization promoted reduction of the fiber area (43 percent, P < 0.05) and increased the connective tissue (200 percent, P < 0.05). The orthosis model was efficient comparing with another alternative immobilization model, like plaster casts, in promoting skeletal muscle alterations, indicating that it could be used as a new model in other studies related to muscle disuse.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Aparelhos Ortopédicos
/
Articulações Tarsianas
/
Resinas Acrílicas
/
Elevação dos Membros Posteriores
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil