Characterization of a specific antibody to the rat angiotensin II AT1 receptor.
J Histochem Cytochem
; 47(4): 507-16, 1999 Apr.
Article
em En
| MEDLINE
| ID: mdl-10082752
ABSTRACT
We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT1 receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT1 receptor and AT1 receptor immunoreactivity.(J Histochem Cytochem 47507-515, 1999)
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Angiotensina
/
Córtex Suprarrenal
Limite:
Animals
Idioma:
En
Revista:
J Histochem Cytochem
Assunto da revista:
HISTOCITOQUIMICA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Austrália