A bioseparation apparatus with high-pressure fluid injection and fluid sampling.
Anal Biochem
; 269(2): 223-9, 1999 May 01.
Article
em En
| MEDLINE
| ID: mdl-10221993
ABSTRACT
A novel apparatus in which fluids may be injected and sampled at high pressure is described. Bioseparation applications of the apparatus were demonstrated in three model systems (1) lambdaDNA was eluted under pressure from an anion exchange column into a low-salt (0.25 M) buffer, thereby eliminating conventional time-consuming desalting procedures required for downstream analysis of the DNA; (2) RNA was separated under pressure from a RNA/DNA mixture, thereby enabling rapid differential preparation of nucleic acids; and (3) an antibody was purified from a protein mixture by affinity capture at one pressure and dissociation from the antigen binding partner at a second pressure, thereby enabling the immunoreactivities of both antibody and antigen to be preserved during the separation process.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioquímica
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos