Field amplified injection in the presence of salts for capillary electrophoresis.

ABSTRACT

Salts in the sample are detrimental to the stacking by the field-amplified injection. However, physiological samples often contain salts at levels of about 1% which can diminish the peak height or cause band spreading instead of stacking. Using different analytes which contain salts, we demonstrate that the presence of acetonitrile at 66% in the sample reverses the deleterious effect of salts and favors the stacking by the electrokinetic injection. The advantage of this type of stacking is that it favors certain analytes over others and it can give, in some instances, better theoretical plate numbers.