An antibody-lectin sandwich assay for quantifying protein glycoforms.

ABSTRACT

We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein's glycoforms by simply substituting the antibody and lectin with specific alternatives.