Identification and characterisation of novel human Y-chromosomal microsatellites from sequence database information.
Nucleic Acids Res
; 28(2): e8, 2000 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-10606676
ABSTRACT
1.33 Mb of sequence from the human Y chromosome was searched for tri- to hexanucleotide microsatellites. Twenty loci containing a stretch of eight or more repeat units with complete repeat sequence homo-geneity were found, 18 of which were novel. Six loci (one tri-, four tetra- and one pentanucleotide) were assembled into a single multiplex reaction and their degree of polymorphism was investigated in a sample of 278 males from Pakistan. Diversities of the individual loci ranged from 0.064 to 0.727 in Pakistan, while the haplotype diversity was 0.971. One population, the Hazara, showed particularly low diversity, with predominantly two haplotypes. As the sequence builds up in the databases, direct methods such as this will replace more biased and technically demanding indirect methods for the isolation of microsatellites.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromossomo Y
/
Bases de Dados Factuais
/
Repetições de Microssatélites
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Reino Unido