Single-molecule identification by spectrally and time-resolved fluorescence detection
Anal Chem
; 72(3): 443-7, 2000 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-10695126
ABSTRACT
A method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined methodology. Monte Carlo simulations show that less than 500 photons are needed to assign an observed single molecule to one out of four species with a confidence level higher than 99.9%. We show that single dye molecules of four different dyes embedded in a polymer film can be identified with time-correlated single-photon counting spectrally resolved in two channels.
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01-internacional
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MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2000
Tipo de documento:
Article
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