Direct high-performance liquid chromatographic determination of the enantiomeric purity of levodopa and methyldopa: comparison with pharmacopoeial polarimetric methods.
J Pharm Biomed Anal
; 19(3-4): 555-67, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10704122
ABSTRACT
Chiral high-performance liquid chromatography was employed for determination of the enantiomeric purity of levodopa and methyldopa. The determination of D-DOPA in levodopa was accomplished using a chiral ligand-exchange chromatograpy with an ordinary C18 column and a chiral mobile phase containing N,N-dimethyl-L-phenylalanine and Cu(II) acetate or by means of LC on a teicoplanin column in conjunction with ethanol-water (6535, v/v). Both methods gave good performance, however, the latter was faster and more convenient and suitable for routine analyses. For the determination of D-methyldopa a LC method based on the use of a teicoplanin column in polar organic mode with methanol-acetic acid-triethylamine (1,0000.050.05, v/v/v) mobile phase was developed. The precision, accuracy, linearity and selectivity were satisfactory. In comparison with pharmacopoeial polarimetric methods (according to the European Pharmacopoeia and the Pharmacopoea Bohemoslovaca), the LC methods proved to be much more sensitive giving detection limits 0.04% of D-DOPA and 0.3% of D-methyldopa.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Farmacopeias como Assunto
/
Levodopa
/
Metildopa
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
República Tcheca