High-fat diets suppress CD3 and CD25 expression on the surface of murine lymphocytes.
Nutrition
; 16(4): 278-83, 2000 Apr.
Article
em En
| MEDLINE
| ID: mdl-10758364
ABSTRACT
To determine the effects of dietary fats on surface antigen expression, we tested the effects of amount and type of dietary fat on murine lymphocytes. Mice were fed diets with 12 en%, 23 en%, or 47 en% fat containing coconut, olive, safflower, or linseed oil. After 2 wk of ad libitum feeding, the mice were killed and splenic lymphocytes were harvested. Lymphocytes were incubated with fluorescent-tagged monoclonal antibodies and assayed for mean and total surface expression using flow cytometry. Our results show that high-fat (47 en%) diets suppress expression of CD3 and CD25 antigens. We also found that linseed-oil diets suppress expression of CD11a but enhance expression of CD25 antigens. Both CD3 and CD25 are critical for lymphocyte activation, and we conclude that immunosuppression associated with high-fat diets may be associated with suppression of these surface antigens.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Gorduras na Dieta
/
Linfócitos T
/
Receptores de Complemento 3b
/
Complexo CD3
Limite:
Animals
Idioma:
En
Revista:
Nutrition
Assunto da revista:
CIENCIAS DA NUTRICAO
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos