Adenovirus-mediated combination suicide and cytokine gene therapy for bladder cancer.
Anticancer Res
; 20(3A): 1359-65, 2000.
Article
em En
| MEDLINE
| ID: mdl-10928044
ABSTRACT
BACKGROUND:
The present study tests the hypothesis that adenovirus-mediated transfer of murine IL-2 (ADV/RSV-mIL-2) alone or in combination with HSV-tk + GCV will improve antitumorigenic response in the murine MBT-2 model. MATERIALS ANDMETHODS:
mIL-2 production and toxicity were determined in vitro using an ELISA and a cell proliferation assay. Tumor-bearing animals were randomly assigned into four treatment groups and directly injected with combinations of ADV/RSV-tk and ADV/RSV-mIL-2. In a separate experiment, the above-mentioned groups were followed by two subsequent treatments with ADV/RSV-mIL-2.RESULTS:
Transduced MBT-2 cells were able to express mIL-2 in a time and dose dependent fashion. We could not demonstrate any improvement in antitumorigenic response with mIL-2 gene therapy alone or in combination with HSV-tk-suicide gene therapy over HSV-tk suicide gene therapy alone.CONCLUSIONS:
Although ADV/RSV-mIL-2 transduced MBT-2 cells were able to produce large amounts of mIL-2 in vitro, we could not demonstrate significant tumor growth inhibition by adding mIL-2 gene therapy to suicide gene therapy. The growth inhibitory effects of sequential suicide and cytokine gene therapy were transient and not superior to single dose suicide and cytokine gene therapy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
/
Terapia Genética
/
Interleucina-2
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos