Development of an in vivo model for study of intestinal invasion by Salmonella enterica in chickens.
Infect Immun
; 68(12): 7122-5, 2000 Dec.
Article
em En
| MEDLINE
| ID: mdl-11083838
ABSTRACT
An in vivo loop test model for the investigation of the invasiveness of Salmonella enterica in chickens was developed. Ten jejunal loops were made in 10- to 12-week-old Lohman Brown chickens under isofluoran anaesthesia. Salmonella at 5.0 x 10(7) CFU was inoculated into each loop and left for 2 h, followed by a 1-h incubation with gentamicin in order to kill noninvading bacteria. After euthanasia, Salmonella invasiveness was measured as tissue-associated counts relative to a reference strain. The ability of Salmonella invasion was 1 log(10) CFU higher per 42-mm(2) mucosal tissue in the anterior than in the posterior part of jejunum. A statistically significant (P<0.001) sixfold difference in invasiveness was observed between a wild-type S. enterica serotype Typhimurium strain and the corresponding invH mutant. The model was shown to be able to show small differences in invasive capability and allows for comparison of strains tested in different animals, provided that the same reference strain is present in all animals.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Salmonella enterica
/
Jejuno
Limite:
Animals
Idioma:
En
Revista:
Infect Immun
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Dinamarca