New polypeptide components purified from mamba venom.
FEBS Lett
; 491(3): 217-21, 2001 Mar 02.
Article
em En
| MEDLINE
| ID: mdl-11240130
ABSTRACT
New polypeptide components have been isolated from Dendroaspis angusticeps venom using chromatography. Two polypeptides containing 59 and 57 amino acids, called 'DaE1' and 'DaE2' respectively, have been purified to homogeneity and fully sequenced. Spectrometric analysis yielded masses of 6631.5 and 6389.0 Da, respectively. The polypeptides share 98 and 95% identity, respectively, with trypsin inhibitor E (DpE) of Dendroaspis polylepis polylepis. 'DaE' polypeptides inhibit Kv1.1 channels with an IC(50) value in the range of 300 nM. They can be considered as new dendrotoxins, albeit with fairly low affinity as compared to alpha-DTX. 'DaE' polypeptides do not affect Kir2.1 channels.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Elapidae
/
Canais de Potássio Corretores do Fluxo de Internalização
/
Canais de Potássio de Abertura Dependente da Tensão da Membrana
/
Venenos Elapídicos
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Bélgica