Triple-helix formation in the antiparallel binding motif of oligodeoxynucleotides containing N(9)- and N(7)-2-aminopurine deoxynucleosides.
Nucleic Acids Res
; 29(11): 2260-7, 2001 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-11376144
ABSTRACT
Triplex-forming oligodeoxynucleotide 15mers, designed to bind in the antiparallel triple-helical binding motif, containing single substitutions (Z) of the four isomeric alphaN(7)-, betaN(7)-, alphaN(9)- and betaN(9)-2-aminopurine (ap)-deoxyribonucleosides were prepared. Their association with double-stranded DNA targets containing all four natural base pairs (X-Y) opposite the aminopurine residues was determined by quantitative DNase I footprint titration in the absence of monovalent metal cations. The corresponding association constants were found to be in a rather narrow range between 1.0 x 10(6) and 1.3 x 10(8) M(-1). The following relative order in Z x X-Y base-triple stabilities was found Z = alphaN(7)ap T-A > A-T> C-G approximately G-C; Z = betaN(7)ap A-T > C-G > G-C > T-A; Z = alphaN(9)ap A-T = G-C > T-A > C-G; and Z = betaN(9)ap G-C > A-T > C-G > T-A.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Nucleosídeos de Purina
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Suíça