Structure of the human M(2) muscarinic acetylcholine receptor gene and its promoter.
Gene
; 271(1): 87-92, 2001 Jun 13.
Article
em En
| MEDLINE
| ID: mdl-11410369
ABSTRACT
The M(2) muscarinic receptor inhibits the release of acetylcholine from cholinergic fibers in the lungs and elsewhere. In airway parasympathetic neurons, M(2) receptor expression is decreased by viral infections and by interferon-gamma, increasing actylcholine release. Dexamethasone increases M(2) receptor expression, decreasing acetylcholine release. We carried out 5' rapid amplification of cDNA ends beginning with mRNA from human heart and IMR32 human neuroblastoma cells. This demonstrated a 5' UTR of 100 BP, corresponding to two sequences on chromosome 7, separated by a 22.6 kB intron. The splice acceptor site is at -45 relative to the initiating atg. The 3000 BP upstream of 5' RACE product were subcloned into a pGL3 luciferase reporter vector. Deletional constructs were expressed in IMR32 cells. These demonstrated that 412 BP provided full expression of the reporter gene, and suggested a repressor element between -1848 and -1510.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores Muscarínicos
/
Regiões Promotoras Genéticas
/
Genes
Limite:
Humans
Idioma:
En
Revista:
Gene
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos