Restoration of abated T cell stimulation activity of mature dendritic cells.
Biochem Biophys Res Commun
; 285(3): 594-7, 2001 Jul 20.
Article
em En
| MEDLINE
| ID: mdl-11453633
ABSTRACT
Freshly isolated and cultured mature dendritic cells (DC) rapidly lose their high capacity to activate T cells. This diminished T cell activation activity is due, in part, to dramatically reduced expression of the costimulation ligands B7-1 (CD80) and B7-2 (CD86). Here we show that cultivation of mature DC with the lymphokines TNFalpha, GM-CSF, or TNFalpha plus GM-CSF, fully restored and enhanced their T-cell stimulation activity by two to threefold, four to fivefold, or six to sevenfold, respectively. The restored T-cell stimulation activity was directly correlated with induced and increased levels of CD80 (16-fold) and CD86 (10-fold) expression by the lymphokine-cultured DC. These results should facilitate many current DC-vaccination efforts towards generating more potent T cell responses.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
/
Linfócitos T
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos