Immunodiagnosis of pneumococcal meningitis using Dot-enzyme-linked immunosorbent assay.
J Trop Pediatr
; 47(5): 288-90, 2001 10.
Article
em En
| MEDLINE
| ID: mdl-11695728
ABSTRACT
A diagnosis of bacterial meningitis requires isolation of the pathogen from cerebrospinal fluid (CSF). However, cultures of CSF are usually insensitive, thus, in the majority of patients, the etiology is rarely determined. A total of 90 CSF samples from pediatric patients with clinical diagnosis of bacterial meningitis were evaluated by Dot-ELISA. This method was standardized in order to detect pneumococcal polysaccharide antigen in CSF samples previously treated with 0.1 M EDTA and dotted on nitrocellulose membrane strips. Pneumococcal omniserum diluted 1200 was employed for pneumococcal antigen detection. Dot-ELISA showed relative indices of 100 and 90 per cent for sensitivity and specificity, respectively. This method is cheaper than counter immunoelectrophoresis for pneumococcal antigen detection.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptococcus pneumoniae
/
Ensaio de Imunoadsorção Enzimática
/
Meningite Pneumocócica
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Child
/
Humans
Idioma:
En
Revista:
J Trop Pediatr
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Brasil