The enantioselective determination of chlorpheniramine and its major metabolites in human plasma using chiral chromatography on a beta-cyclodextrin chiral stationary phase and mass spectrometric detection.
J Pharm Biomed Anal
; 27(3-4): 479-88, 2002 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-11755749
ABSTRACT
A sensitive enantioselective high-performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of plasma concentrations of (-)(R)- and (+)(S)-chlorpheniramine (CP) and their metabolites, desmethyl-chlorpheniramine (DCP), didesmethyl-chorpheniramine (DDCP) and chlorpheniramine N-oxide (CPNO). Enantioselective separations were achieved on a beta-cyclodextrin chiral stationary phase (CYCLOBOND I 2000) with a mobile phase consisting of diethylamine acetate (0.25%, pH 4.4)methanolacetonitrile [857.57.5, (v/v/v)]and a flow-rate of 0.5 ml/min. For CP, the enantioselectivity (alpha) of the separation was 1.12 with a resolution factor (R(s)) of 1.17. The method was validated for CP by using mass spectroscopy detection (MSD). Concentrations of each enantiomer could be measured down to 125 pg/ml from a 1-ml plasma sample. Extracted calibration curves were linear from 0.13 to 50.00 ng/ml for each enantiomer. The method was applied to samples from two clinical studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clorfeniramina
/
Antialérgicos
/
Ciclodextrinas
/
Beta-Ciclodextrinas
Tipo de estudo:
Clinical_trials
/
Diagnostic_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos