Identification of nontuberculous mycobacteria existing in tap water by PCR-restriction fragment length polymorphism.
Appl Environ Microbiol
; 68(6): 3159-61, 2002 Jun.
Article
em En
| MEDLINE
| ID: mdl-12039784
ABSTRACT
This paper presents the finding of the possible cause of the high false-positive rate in acid-fast staining in histological examinations. Using acid-fast staining, culture, and PCR, acid-fast bacilli were detected in 83.7% of 49 hospital tap water samples and nontuberculous mycobacteria (NTM) were detected in 20.4% of the same 49 samples. The 10 NTM isolates were also identified to the species level using PCR-restriction fragment length polymorphism. Our findings indicate that NTM in hospital tap water are the possible cause of false positives in acid-fast staining and of nosocomial infection in immunocompromised patients.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
Mycobacterium
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
China