Genetic Contamination of an SJL/J Mouse Colony: Rapid Detection by PCR-based Microsatellite Analysis.
Contemp Top Lab Anim Sci
; 38(2): 54-55, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-12086434
ABSTRACT
Six simple sequence-length polymorphisms (SSLPs), or microsatellites, generated by polymerase chain reaction (PCR), were used to detect genetic contamination of an SJL/J mouse colony in a faster and more economical way than standard immunologic and biochemical markers. The marker set was chosen to assess SJL authenticity and to distinguish a possible outcross with BALB/c, the only other albino strain maintained at the facility. Evidence of genetic contamination as a result of an outcross with BALB/c was found, and the results were ready within a workday.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Contemp Top Lab Anim Sci
Assunto da revista:
MEDICINA VETERINARIA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Argentina