Osmotic stress sensitizes naturally resistant cells to TNF-alpha-induced apoptosis.
Cell Death Differ
; 9(10): 1090-8, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12232797
ABSTRACT
Most cells are naturally resistant to TNF-alpha-induced cell death and become sensitized when NF-kappaB transactivation is blocked or in the presence of protein synthesis inhibitors that prevent the expression of anti-apoptotic genes. In this report we analyzed the role of osmotic stress on TNF-alpha-induced cell death. We found that it sensitizes the naturally resistant HeLa cells to TNF-alpha-induced apoptosis, with the involvement of an increase in the activity of several kinases, the inhibition of Bcl-2 expression, and a late increase on NF-kappaB activation. Cell death occurs regardless of the enhanced NF-kappaB activity, whose inhibition produces an increase in apoptosis. The inhibition of p38 kinase, also involved in NF-kappaB activation, significantly increases the effect of osmotic stress on TNF-alpha-induced cell death.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
/
NF-kappa B
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Sistema de Sinalização das MAP Quinases
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Células Eucarióticas
Limite:
Humans
Idioma:
En
Revista:
Cell Death Differ
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Argentina