In situ observation of mobility and anchoring of PKCbetaI in plasma membrane.
FEBS Lett
; 541(1-3): 126-31, 2003 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-12706832
ABSTRACT
We employed fluorescence correlation spectroscopy (FCS) to analyze the characteristics of biomolecules in living cells. Protein kinase C (PKC) changes its subcellular localization from cytosol to the plasma membrane by its ligand. Using FCS, we found PKCbetaI labeled with enhanced green fluorescent protein freely diffusing in cytosol. Upon 12-O-tetradecanoylphorbol-13-acetate activation, a large part of PKCbetaI is anchored in the plasma membrane but some PKCbetaI still moves freely near the plasma membrane. These results indicate that a diffusion-driven transport mechanism is appropriate for the molecular mechanism of the PKCbetaI localization change.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteína Quinase C
/
Membrana Celular
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Japão