Production of knockout rats using ENU mutagenesis and a yeast-based screening assay.
Nat Biotechnol
; 21(6): 645-51, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-12754522
ABSTRACT
The rat is a widely used model in biomedical research and is often the preferred rodent model in many areas of physiological and pathobiological research. Although many genetic tools are available for the rat, methods to produce gene-disrupted knockout rats are greatly needed. In this study, we developed protocols for creating N-ethyl-N-nitrosourea (ENU)-induced germline mutations in several rat strains. F1 preweanling pups from mutagenized Sprague Dawley (SD) male rats were then screened for functional mutations in Brca1 and Brca2 using a yeast gap-repair, ADE2-reporter truncation assay. We produced knockout rats for each of these two breast cancer suppressor genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ratos Mutantes
/
Regulação da Expressão Gênica
/
Mutagênese Sítio-Dirigida
/
Marcação de Genes
/
Etilnitrosoureia
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Limite:
Animals
Idioma:
En
Revista:
Nat Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos