Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.
Transgenic Res
; 1(2): 101-5, 1992 Mar.
Article
em En
| MEDLINE
| ID: mdl-1284480
ABSTRACT
We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisely disrupts exon 10, the site of the major mutation in patients with cystic fibrosis. The targeted cells are pluripotent and competent to form chimaeras.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Fibrose Cística
/
Embrião de Mamíferos
/
Proteínas de Membrana
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Transgenic Res
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Reino Unido