Absence of the LiTat 1.3 (CATT antigen) gene in Trypanosoma brucei gambiense stocks from Cameroon.
Acta Trop
; 51(2): 123-34, 1992 Jun.
Article
em En
| MEDLINE
| ID: mdl-1354930
ABSTRACT
Antibodies to the variable antigen type (VAT) designated LiTat 1.3 are common in sera from parasitologically confirmed patients with gambian sleeping sickness. For this reason, LiTat 1.3 has been considered a suitable antigen for detecting Trypanosoma brucei gambiense in the Card Agglutination Test for Trypanosomiasis (CATT; Testryp-CATT, Smith Kline-RIT). However, surveys in the T.b. gambiense endemic focus of Fontem in Cameroon have suggested that expression of LiTat 1.3 might be rare or absent. We show here that the gene for LiTat 1.3 was indeed absent from some T.b. gambiense stocks isolated from this focus, and a LiTat 1.3-like gene was present in others. The divergent gene differed from the cloned version of LiTat 1.3. In addition, antibodies to LiTat 1.3 could not be detected in rabbits infected with either of the two kinds of T.b. gambiense from the Fontem area. We suggest that the absence of LiTat 1.3 expression in this focus may have important implications for the epidemiology and control of sleeping sickness, especially if heavy reliance is placed on the CATT.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma brucei gambiense
/
Anticorpos Antiprotozoários
/
Antígenos de Protozoários
Limite:
Animals
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Acta Trop
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Reino Unido