Erythropoietic progenitor cells from premature neonates studied using a validated serum-deprived medium. Evidence that peripheral blood BFU-E from premature neonates are increased in number and are highly dependent upon burst promoting activity for in vitro growth.
Cytotechnology
; 6(3): 241-6, 1991 Jul.
Article
em En
| MEDLINE
| ID: mdl-1367667
ABSTRACT
We have examined a serum-deprived culture system in order to verify that it is suitable for the study of burst forming unit erythroid (BFU-E) progenitor cells from premature neonates. Optimum growth of BFU-E from premature neonates was observed with each media constituent using the same concentration as that previously described for adult subjects. Growth of immature BFU-E from premature neonates were highly dependent upon a source of Burst Promoting Activity and mature BFU-E derived colonies emerged at day 12 compared to day 14 in adults. Our preliminary results with the validated medium suggest that premature infants have increased peripheral blood concentrations of BFU-E compared to healthy adult controls.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Precursoras Eritroides
/
Meios de Cultura Livres de Soro
Limite:
Adult
/
Humans
/
Infant
/
Middle aged
/
Newborn
Idioma:
En
Revista:
Cytotechnology
Assunto da revista:
BIOTECNOLOGIA
/
GENETICA
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Reino Unido