YAC-assisted cloning of a putative G-protein mapping to the MHC class I region.
Genomics
; 14(4): 857-62, 1992 Dec.
Article
em En
| MEDLINE
| ID: mdl-1478666
ABSTRACT
We report the successful use of whole yeast artificial chromosomes (YACs) as probes for direct positional cloning of novel expressed sequences in a given genomic fragment. The class I region of the human major histocompatibility complex, in particular the chromosomal fragment spanning the HLA-E locus, was investigated. The screening of a cDNA library with a 210-kb-long YAC clone led to the identification of a new gene, positionally conserved in the major histocompatibility complex of the mouse genome and encoding a putative GTP binding protein. Although its precise function remains unknown, the interspecies conservation of both sequence and map position suggests a regulatory or functional link with the histocompatibility cluster.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes MHC Classe I
/
Proteínas de Ligação ao GTP
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França