Fetal ascitic fluid: a new source of lymphocytes for rapid chromosomal analysis.
Obstet Gynecol
; 80(3 Pt 2): 533-5, 1992 Sep.
Article
em En
| MEDLINE
| ID: mdl-1495731
ABSTRACT
BACKGROUND:
The cells found in ascites can be processed like amniotic fluid for fetal karyotyping. We have characterized these cells and used them for a rapid cytogenetic result. CASES Three patients presented with massive fetal ascites detected by sonography. Samples of ascitic fluid were obtained at fetal paracentesis. Cells from the fluid were cultured using standard methods for fetal blood, and were compared with fetal blood lymphocytes and amniocytes. The length of time in culture, chromosome morphology, and mitotic index of ascitic fluid cells were equivalent to those of fetal blood. In the third case, we performed immunophenotyping on the ascitic fluid cells.CONCLUSION:
Ascitic fluid contains lymphocytes that permit rapid chromosomal analysis within 96 hours.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Diagnóstico Pré-Natal
/
Líquido Ascítico
/
Aberrações Cromossômicas
/
Doenças Fetais
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Obstet Gynecol
Ano de publicação:
1992
Tipo de documento:
Article