Mouse embryonic stem cell sorting for the generation of transgenic mice by sedimentation field-flow fractionation.
Anal Chem
; 76(6): 1580-5, 2004 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-15018554
ABSTRACT
Mouse embryonic stem (ES) cells are an important tool for generation of transgenic mice and genetically modified mice. A rapid and efficient separation of ES cells that respects cell integrity, viability, and their developmental potential while also allowing purified ES fraction collection under sterile conditions might be of great interest to facilitate the generation of chimeric animals. In this study, we demonstrated for the first time the effectiveness of a sedimentation field-flow fractionation (SdFFF) cell sorter to provide, with a characteristic DNA content, a purified ES cell fraction and with a high in vivo developmental potential to prepare transgenic mice by generation of chimeras having a high percentage of chimerism.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Camundongos Transgênicos
/
Separação Celular
/
Quimera
/
Fracionamento por Campo e Fluxo
/
Embrião de Mamíferos
Limite:
Animals
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
França