Pseudomonas aeruginosa from canine otitis externa exhibit a quorum sensing deficiency.
Vet Microbiol
; 99(2): 121-9, 2004 Apr 05.
Article
em En
| MEDLINE
| ID: mdl-15019103
ABSTRACT
Pseudomonas aeruginosa LasB elastase gene (lasB) transcription depends on cell density-dependent quorum-sensing mechanisms of gene activation. Previously, we collected several non-mucoid P. aeruginosa veterinary isolates and showed that the total matrix protease phenotype was similar for isolates regardless of host and site of isolation. In contrast, isolates from chronic canine ear infections (otitis externa) were significantly more likely to exhibit less elastase activity as measured by elastin Congo red than from any other site [Clin. Diag. Lab. Immun. 8 (2001) 632]. In this study, we found that the elastase deficiency phenotype is stable upon passage in broth culture. Transcript amplification analyses indicated that the elastase deficiency appears to be strain-specific, with each isolate exhibiting a unique expression profile relative to strain PAO1. Although a number of strain-specific transcriptional differences were observed, the overall pattern that emerges is a quorum sensing deficiency among canine ear P. aeruginosa isolates.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Otite Externa
/
Pseudomonas aeruginosa
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Infecções por Pseudomonas
/
Proteínas de Bactérias
/
Metaloendopeptidases
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Elastase Pancreática
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Regulação Bacteriana da Expressão Gênica
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Regulação Enzimológica da Expressão Gênica
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Doenças do Cão
Limite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos