[Cloning and sequence analysis of the genome of a strain of duck hepatitis B virus].
Di Yi Jun Yi Da Xue Xue Bao
; 24(4): 404-7, 2004 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-15090309
ABSTRACT
OBJECTIVE:
To clone and analyze the genome of a duck hepatitis B virus (DHBV) strain isolated from the ducks found in the local area.METHODS:
The complete genome of DHBV was amplified by PCR prior to cloning into T vector and sequencing, with also homology and phylogenetic analyses.RESULT:
Genome comparison of an isolated DHBV strain and 16 DHBV strains in GenBank revealed a homology of 89.4% to 99.3% at the nucleotide level, and the amino acid identity of the 3 open reading frames showed that the P region harbored more obvious variations than the other regions.CONCLUSION:
The isolated DHBV strain might belong to a subtype of the virus found in the western countries.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Vírus da Hepatite B do Pato
Limite:
Animals
Idioma:
Zh
Revista:
Di Yi Jun Yi Da Xue Xue Bao
Assunto da revista:
MEDICINA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China