Changes in drug 13C NMR chemical shifts as a tool for monitoring interactions with DNA.
Biophys Chem
; 109(3): 333-44, 2004 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-15110931
ABSTRACT
The antibiotic drug, netropsin, was complexed with the DNA oligonucleotide duplex [d(GGTATACC)]2 to monitor drug 13C NMR chemical shifts changes. The binding mode of netropsin to the minor groove of DNA is well-known, and served as a good model for evaluating the relative sensitivity of 13C chemical shifts to hydrogen bonding. Large downfield shifts were observed for four resonances of carbons that neighbor sites which are known to form hydrogen bond interactions with the DNA minor groove. Many of the remaining resonances of netropsin exhibit shielding or relatively smaller deshielding changes. Based on the model system presented here, large deshielding NMR shift changes of a ligand upon macromolecule binding can likely be attributed to hydrogen bond formation at nearby sites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Ressonância Magnética Nuclear Biomolecular
/
Antibacterianos
/
Netropsina
Idioma:
En
Revista:
Biophys Chem
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos