[Determination of tyrosine- and tryptophan-containing peptides in plasma by absorption in UV spectral region]. / Opredelenie tirozin- i triptofansoderzhashchikh peptidov v plazme krovi po pogloshcheniiu v UF-oblasti spektra.
Klin Lab Diagn
; (3): 12-6, 2004 Mar.
Article
em Ru
| MEDLINE
| ID: mdl-15137197
ABSTRACT
A method for determining the cumulative concentration of tyrosine- and tryptophan-containing peptides (TSP and TPP) in blood plasma 0.2 ml of blood plasma is added 0.1 ml of 1.8 M chloric acid, the sample is centrifuged, the supernatant (0.2 ml) is added 1.8 ml of 0.7 M NaOH and the optic density is measured at 290 nm in the quarts or plastic flask. Equations were derived to calculate separately the concentrations of TSP and TPP, whose normal values are 1.28 +/- 0.31 and 0.25 +/- 0.06 mmol/l, respectively. The increasing total content of peptides in thyrotoxicosis was shown to be mainly conditioned by a higher TSP concentration (a 2.4-fold increase). Therefore, TSP is a more sensitive marker in case of endogenous intoxication versus the generally known index of the cumulative content of oligopeptides.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Tirosina
/
Triptofano
Limite:
Humans
Idioma:
Ru
Revista:
Klin Lab Diagn
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2004
Tipo de documento:
Article