Role of pilA, an essential regulatory gene of Neisseria gonorrhoeae, in the stress response.
J Bacteriol
; 174(18): 5978-81, 1992 Sep.
Article
em En
| MEDLINE
| ID: mdl-1522071
ABSTRACT
Sequence analysis has shown that PilA, a transcriptional regulator of pilin gene expression in Neisseria gonorrhoeae, has extensive homology with the 54-kDa protein of the signal recognition particle of eukaryotes and its receptor, as well as with two proteins of Escherichia coli, FtsY and Ffh, which have been proposed to be a part of a signal recognition particle-like apparatus. We tested the putative role of PilA in protein export in N. gonorrhoeae and did not find any effect. However, we did observe induction of a heat shock response and a previously described slow-growth phenotype when PilA function was impaired. We also examined the interference of pilA expression in E. coli with the function of the products of ftsY and ffh and observed an accumulation of pre-beta-lactamase. We argue against a direct role for PilA in protein export in gonococci and propose instead that PilA is involved in the modulation of cell growth rate in response to different environmental conditions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Regulação Bacteriana da Expressão Gênica
/
Genes Reguladores
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Proteínas de Ligação a DNA
/
Neisseria gonorrhoeae
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França