Detection of stanozolol and its metabolites in equine urine by liquid chromatography-electrospray ionization ion trap mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci
; 811(1): 75-83, 2004 Nov 05.
Article
em En
| MEDLINE
| ID: mdl-15458725
ABSTRACT
The equine phase I and phase II metabolism of the synthetic anabolic steroid stanozolol was investigated following its administration by intramuscular injection to a thoroughbred gelding. The major phase I biotransformations were hydroxylation at C16 and one other site, while phase II metabolism in the form of sulfate and beta-glucuronide conjugation was extensive. An analytical procedure was developed for the detection of stanozolol and its metabolites in equine urine using solid phase extraction, acid solvolysis of phase II conjugates and analysis by positive ion electrospray ionization ion trap LC-MS.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estanozolol
/
Espectrometria de Massas por Ionização por Electrospray
/
Anabolizantes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Austrália