An improved protocol for pulsed-field gel electrophoresis typing of Clostridium difficile.
J Med Microbiol
; 54(Pt 2): 155-157, 2005 Feb.
Article
em En
| MEDLINE
| ID: mdl-15673509
ABSTRACT
Pulsed-field gel electrophoresis (PFGE) is the 'gold standard' technique for bacterial typing and has proved to be discriminatory and reproducible for typing Clostridium difficile. Nevertheless, a high proportion of strains are non-typable by this technique due to the degradation of the DNA during the process. The introduction of several modifications in the PFGE standard procedure increased typability from 40% (90 isolates) to 100% (220 isolates) while maintaining the high degree of discrimination and reproducibility of the technique.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clostridioides difficile
/
Técnicas de Tipagem Bacteriana
/
Eletroforese em Gel de Campo Pulsado
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Espanha