Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos.
Theriogenology
; 63(5): 1308-19, 2005 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-15725439
ABSTRACT
Experiments were conducted to determine viability of equine embryos in vivo after vitrification. In a preliminary study (Experiment 1), embryos were exposed in three steps to vitrification solutions containing increasing concentrations of ethylene glycol and glycerol (EG/G); the final vitrification solution was 3.4 M glycerol + 4.6 M ethylene glycol in a base medium of phosphate-buffered saline. Embryos were warmed in a two-step dilution and transferred into uteri of recipients. No pregnancies were observed after transfer of blastocysts >300 microm (n = 3). Transfer of morulae or blastocysts < or = 300 microm resulted in four embryonic vesicles (4/6, 67%). In a second experiment, embryo recovery per ovulation was similar for collections on Day 6(28/36, 78%) versus Days 7 and 8(30/48, 62%). Embryos < or = 300 and >300 microm were vitrified, thawed and transferred as in Experiment 1. Some embryos < or = 300 microm were also transferred using a direct-transfer procedure (DT). Embryo development rates to Day 16 were not different for embryos < or = 300 microm that were treated as in Experiment 1(10/22, 46%) or transferred by DT (16/26, 62%). Embryos > 300 microm (n = 19) did not produce embryonic vesicles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Transferência Embrionária
/
Cavalos
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Theriogenology
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos