Human microvascular endothelial cells from different fetal organs demonstrate organ-specific CAM expression.
Exp Cell Res
; 308(2): 273-82, 2005 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-15936757
ABSTRACT
In this work, we isolated and produced long-term cultures of human fetal endothelial cells (fECs) deriving from different organs of the same 12-week-old embryos. Highly pure endothelium cultures were obtained from specimens of brain, heart, lung, liver, aorta and kidney by using magnetic microspheres coated with CD31 or CD34 specific endothelial antibodies. The endothelial nature of these cells was confirmed by the presence of von Willebrand Factor (vWf), Flk-1/VEGFR2 and CD31. The fECs cultures showed organ-specific differences as regards to the morphological appearance, the growth rate and the expression of cellular adhesion molecules (CAMs) before or after stimulation by the inflammatory cytokines IL-1beta and TNF-alpha. For instance, TNF-alpha showed a specific effect on fetal heart ECs by stimulating E-selectin expression. Our findings indicate that fECs may represent an innovative tool to study differences among ECs of different vascular districts of the same individual, thus increasing the possibility to compare many pathological aspects of human adult and fetal microvasculature.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vísceras
/
Endotélio Vascular
/
Moléculas de Adesão Celular
/
Neovascularização Fisiológica
/
Microcirculação
Limite:
Humans
Idioma:
En
Revista:
Exp Cell Res
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Itália