Maternal origin of inflammatory leukocytes in preterm fetal membranes, shown by fluorescence in situ hybridisation.
Placenta
; 26(8-9): 672-7, 2005.
Article
em En
| MEDLINE
| ID: mdl-16085046
ABSTRACT
The aim of this study was to determine the maternal or fetal origin of inflammatory leukocytes in fetal membranes from cases of chorioamnionitis. Fetal membranes were collected from male preterm infants and chorioamnionitis was diagnosed histologically. Fluorescence in situ hybridisation for X and Y chromosomes was used to determine the gender of infiltrating leukocytes in the chorion and amnion. Leukocytes, trophoblast and mesenchymal cells were identified using immunohistochemistry for CD45, cytokeratin-7 and vimentin, respectively. Leukocytes present in the chorion and amnion were labelled XX, indicating maternal origin, and these cells were immunoreactive for the leukocyte marker CD45 but not for vimentin or cytokeratin-7. All other cells in the chorion and amnion were labelled XY and of fetal origin. The results indicated that maternal leukocytes invade the amnion and chorion in chorioamnionitis and we suggest that this is part of the maternal inflammatory response to intrauterine infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corioamnionite
/
Coloração Cromossômica
/
Membranas Extraembrionárias
/
Trabalho de Parto Prematuro
/
Leucócitos
/
Troca Materno-Fetal
Limite:
Adult
/
Female
/
Humans
/
Male
/
Pregnancy
Idioma:
En
Revista:
Placenta
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Reino Unido