[Melanoma cell lines as the basis for antitumor vaccine preparation].
Vestn Ross Akad Med Nauk
; (7): 37-40, 2005.
Article
em Ru
| MEDLINE
| ID: mdl-16107020
ABSTRACT
The aim of the study was to obtain cell lines from tumor samples, and to determine phenotypic cell characteristics in order to choose the optimal line for vaccine preparation. 15 cell lines with stable growth, varying in cultural growth character and cytomorphology, were obtained from samples taken from patients with metastatic skin melanoma. Immunofluorescense method was used to determine the expression of T- and B-lymphocyte markers, antigens of major histocompatibility complex (MHC) class I and II, and CD86 co-stimulating molecule in the cell lines. The expression of melanocyte differentiation antigens and cancer/testicular antigens was evaluated using immunocytochemical assay. The results allowed the authors to distinguish three types of melanoma cell lines according to the expression of MHC molecules MHC-negative; MHC class I positive; MHC classes I and II positive.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Cutâneas
/
Vacinas Anticâncer
/
Melanoma
Limite:
Humans
Idioma:
Ru
Revista:
Vestn Ross Akad Med Nauk
Assunto da revista:
MEDICINA
Ano de publicação:
2005
Tipo de documento:
Article