Sedimentation field flow fractionation monitoring of rice starch amylolysis.
J Chromatogr A
; 1093(1-2): 147-55, 2005 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-16233880
ABSTRACT
Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this work, we investigated the capacity of SdFFF to monitor the native rice starch amylolysis. In order to determine if fractogram changes observed were correlated to granule biophysical modifications which occurred during amylolysis, SdFFF separation was associated with particle size distribution analysis. The results showed that SdFFF is an effective tool to monitor amylolysis of native rice starch. SdFFF analysis was a rapid (less than 10 min), simple and specific method to follow biophysical modifications of starch granules. These results suggested many different applications such as testing series of enzymes and starches. By using sub-population sorting, SdFFF could be also used to better understand starch hydrolysis mechanisms or starch granule structure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Amido
/
Amilases
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
França