Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy.
Appl Environ Microbiol
; 57(9): 2731-4, 1991 Sep.
Article
em En
| MEDLINE
| ID: mdl-16348556
ABSTRACT
Abundance of bacteria and tiny DNA-associated particles in the upper layer of Japanese coastal and offshore waters was evaluated by epifluorescence microscopy with 0.015-mum-pore-size Nuclepore filters. The number of tiny DNA-associated particles was compared with the abundance of virus particles estimated by transmission electron microscopy. Although a large variation in virus abundance (1.2 x 10 to 35 x 10 ml) was obtained with the transmission electron microscopy method, the ratio of 4',6-diamidino-2-phenylindole-reactive tiny particles to viruses was in a rather narrow range (1.0 to 1.6), indicating that the majority of the tiny DNA-associated particles identified by epifluorescence microscopy were actually virus particles. This result implies the possibility of using epifluorescence microscopy for the evaluation of virus abundance in marine environments.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Japão