Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells.
J Radiat Res
; 47(1): 19-25, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16571915
ABSTRACT
In the present study, using inhibitors of ceramide synthase (fumonisin B1), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B1 and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia de Células T
/
Ceramidas
/
Apoptose
Limite:
Humans
Idioma:
En
Revista:
J Radiat Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão