Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry.
Appl Microbiol Biotechnol
; 74(2): 474-83, 2007 Feb.
Article
em En
| MEDLINE
| ID: mdl-17021868
ABSTRACT
We examined hydrogen production from a dairy cow waste slurry (13.4 g of volatile solids per liter) by batch cultures in a temperature range from 37 to 85 degrees C, using microflora naturally present within the slurry. Without the addition of seed bacteria, hydrogen was produced by simply incubating the slurry, using the microflora within the slurry. Interestingly, two peaks of fermentation temperatures for hydrogen production from the slurry were observed at 60 and 75 degrees C (392 and 248 ml H2 per liter of slurry, respectively). After the termination of the hydrogen evolution, the microflora cultured at 60 degrees C displayed hydrogen-consuming activity, but hydrogen-consuming activity of the microflora cultured at 75 degrees C was not detected, at least for 24 days. At both 60 and 75 degrees C, the main by-product was acetate, and the optimum pH of the slurry for hydrogen production was around neutral. Bacteria related to hydrogen-producing moderate and extreme thermophiles, Clostridium thermocellum and Caldanaerobacter subterraneus, were detected in the slurries cultured at 60 and 75 degrees C, respectively, by denaturing gradient gel electrophoresis analyses, using the V3 region of 16S rDNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias Anaeróbias
/
Temperatura Alta
/
Hidrogênio
/
Esterco
Limite:
Animals
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão