[Correlation of DNA-PK activity with anti-cancer drug-sensitivity in human gliomas].
Zhonghua Zhong Liu Za Zhi
; 28(5): 342-4, 2006 May.
Article
em Zh
| MEDLINE
| ID: mdl-17044996
ABSTRACT
OBJECTIVE:
To investigate the relationship between DNA-dependent protein kinase (DNA-PK) activity and anti-cancer drug sensitivity in human glioma tissues.METHODS:
Human glioma specimens were primarily cultured and its sensitivity to several anti-cancer drugs were evaluated by MTT assay. Nuclear protein was extracted from the glioma sample of the same patient and its DNA-PK activity was determined by a biotinylated DNA-PK assay with p53-derived peptide as a specific substrate.RESULTS:
DNA-PK activity varied widely among these glioma samples. Of all 36 samples, 16 showed higher DNA-PK activity (relative activity > or = 0.40) and 20 samples with lower DNA-PK activity (relative activity < 0.40). The gliomas sensitive to DDP and VCR as evaluated by inhibition rate (IR > or = 50%) under plasma peak concentration (PPC) showed lower DNA-PK activity than the resistant ones (IR < 50%) (t = -3.445, P < 0.01). Furthermore, the gliomas with higher DNA-PK activity showed lower inhibition rate (IR < 50%) than those with lower DNA-PK activity ones (t = -2.145, P < 0.05).CONCLUSION:
DNA-PK activity is significantly associated with anti-cancer drug sensitivity to DDP and VCR in human gliomas. DNA-PK activity could be used as a new biomarker for the chemotherapy sensitivity of human gliomas.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vincristina
/
Proteínas Nucleares
/
Cisplatino
/
Resistencia a Medicamentos Antineoplásicos
/
Proteína Quinase Ativada por DNA
/
Glioma
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Zhong Liu Za Zhi
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China