A method for detection of human papillomavirus DNA in stored slides of stained cervical smears.
Pathol Res Pract
; 186(5): 666-7, 1990 Oct.
Article
em En
| MEDLINE
| ID: mdl-1705024
ABSTRACT
A simple method was developed for extraction of DNA from stored slides of Papanicolaou stained cervical smears. HPV type 16 DNA was detected by DNA hybridization in 7/10 samples from known HPV-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. Using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Preservação Biológica
/
Esfregaço Vaginal
/
DNA Viral
/
Teste de Papanicolaou
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Pathol Res Pract
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Itália