Unexpected regioselectivity of Humicola insolens Cel7B glycosynthase mutants.
Carbohydr Res
; 342(5): 710-6, 2007 Apr 09.
Article
em En
| MEDLINE
| ID: mdl-17224137
ABSTRACT
Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl N(I)-acetyl-2(II)-azido-beta-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a beta-(1-->4) linkage between the two disaccharides, while single mutant Cel7B E197A and triple mutant Cel7B E197A H209A A211T produce predominantly the beta-(1-->3)-linked tetrasaccharide. This result constitutes the first report of the modulation of the regioselectivity through site-directed mutagenesis for an endoglycosynthase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Glicosídeo Hidrolases
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França