[Cloning and characterization of genes differentially expressed in human dental pulp cells and gingival fibroblasts].
Hua Xi Kou Qiang Yi Xue Za Zhi
; 25(1): 75-8, 2007 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-17375585
ABSTRACT
OBJECTIVE:
To study the biological properties of human dental pulp cells (HDPC) by cloning and analysis of genes differentially expressed in HDPC in comparison with human gingival fibroblasts (HGF).METHODS:
HDPC and HGF were cultured and identified by immunocytochemistry. HPDC and HGF subtractive cDNA library was established by PCR-based modified subtractive hybridization, genes differentially expressed by HPDC were cloned, sequenced and compared to find homogeneous sequence in GenBank by BLAST.RESULTS:
Cloning and sequencing analysis indicate 12 genes differentially expressed were obtained, in which two were unknown genes. Among the 10 known genes, 4 were related to signal transduction, 2 were related to trans-membrane transportation (both cell membrane and nuclear membrane), and 2 were related to RNA splicing mechanisms.CONCLUSION:
The biological properties of HPDC are determined by the differential expression of some genes and the growth and differentiation of HPDC are associated to the dynamic protein synthesis and secretion activities of the cell.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Clonagem de Organismos
Limite:
Humans
Idioma:
Zh
Revista:
Hua Xi Kou Qiang Yi Xue Za Zhi
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China