Corneal virulence of Pseudomonas aeruginosa elastase B and alkaline protease produced by Pseudomonas putida.
Curr Eye Res
; 32(4): 373-86, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17453960
ABSTRACT
PURPOSE:
To measure the specific virulence contributions of two Pseudomonas aeruginosa proteases, elastase B and alkaline protease, when expressed separately by Pseudomonas putida in a rabbit model of bacterial keratitis.METHODS:
P. putida KT2440 was transformed with plasmids that enabled the extracellular production of either elastase or alkaline protease. Protease expression was confirmed by zymography and immunoblotting. P. putida expressing elastase, alkaline protease, or vector alone was injected intrastromally (10(3) colony forming units [CFU]) into rabbit corneas (n=6). Infected eyes were graded by slit-lamp examination (SLE) at 20, 24, 28, and 32 hr postinfection (PI). Rabbits were sacrificed at 33 hr PI, and the log CFU (+/-SEM) per cornea was determined.RESULTS:
SLE scores for eyes infected with P. putida producing elastase were significantly higher than those infected with vector alone at all time points (p
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Pseudomonas aeruginosa
/
Infecções por Pseudomonas
/
Proteínas de Bactérias
/
Metaloendopeptidases
/
Pseudomonas putida
/
Córnea
/
Ceratite
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos