[In vitro studies on asexual embryos and regenerated plantlets obtained from leaf organ of Panax notoginseng].
Zhongguo Zhong Yao Za Zhi
; 32(6): 481-3, 2007 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-17552149
ABSTRACT
OBJECTIVE:
To study and improve the tissue culture technology of Panax notoginseng.METHOD:
Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet. RESULT ANDCONCLUSION:
Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Regeneração
/
Folhas de Planta
/
Panax notoginseng
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China